Welcome to Mass Spectrometry and Proteomics

The Mass Spectrometry & Proteomics (MSP) facility located within Clinical & Health Sciences at the University of South Australia provides expertise for Tissue Mass Spectrometry Imaging and proteomic analyses. MSP is a Proteomics Node for Bioplatforms Australia in South Australia that was established under the National Collaborative Research Infrastructure Strategy (NCRIS). We offer all types of proteomic services including quantitative proteomics, phosphoproteomics and glycomics. Furthermore, our facility offers, to a limited extent, lipidomics and metabolomics, in collaboration with the Australian Wine Research Institute (AWRI), which is a Metabolomics Node for Bioplatforms Australia, also located in South Australia.

Bioplatforms Australia’s Vision

Bioplatforms Australia enables Australian life science research by investing in state-of-the-art infrastructure and associated expertise in the specialist fields of genomics, proteomics, metabolomics and bioinformatics. Bioplatforms’ proteomics partners offer leading edge infrastructure, expertise and advanced protein discovery services. A broad range of services are offered including mass spectrometry, monoclonal antibody production and drug screening.

Bioplatforms Australia’s Mission is to support world class research and innovation in the life sciences by:
  • Ensuring broad and efficient access to state-of-the-art biomolecular (‘omic) platform capabilities.
  • Facilitating greater multi-disciplinary integration in basic and translational bioscience.
  • Contributing to research collaborations that address scientific challenges of national significance.
  • Supporting industrial applications of biomolecular technology and scientific collaborations between researchers and industry.
  • Expanding international research networks.
  • Sustaining a national intellectual capability and capacity.

Our People

Instrumentation

Autoflex speed MALDI-ToF/ToF MS (with Biotyper capability)

The autoflex speed matrix assisted laser desorption/ionization (MALDI) Time of Flight (ToF) is a highly sensitive technique that allows multiple applications for comprehensive analyte characterization. The system allows analysis for multiple analytes, including (but not limited to), small molecules, metabolites, lipids, peptides, proteins, and polymers. This MS system is configured as a Biotyper platform to allow Fast and reliable identification of microorganisms. MALDI Biotyper systems provide high-speed, high-confidence identification and taxonomical classification of bacteria, yeasts, and fungi. Classification and identification are based on proteomic fingerprinting using high-throughput MALDI-ToF mass spectrometry. Numerous studies have demonstrated the higher accuracy, faster time-to-results, and lower costs provided by MALDI Biotyper systems compared to classical methods.

Ultraflextreme MALDI-ToF/ToF MS

The ultrafleXtreme MS system is designed for and excels in application areas such as Top-Down Proteomics, LC-MALDI, MALDI Imaging, Protein Quantification, Post-Translational Modification Analysis and naturally, Protein Identification. As the MS imaging node of Bioplatforms, the system is supported by the latest hardware for sample preparation (including a Bruker ImagePrep and iMatrixSpray systems) and software (Bruker flexImaging and SCiLS lab Pro MVS) for image generation and statistical evaluation. In MALDI MS Imaging, Images are generated by collecting MALDI data in a virtual grid pattern applied to a sample surface. Thin sections of samples (e.g. fresh frozen or formalin fixed, paraffin embedded tissue) are mounted onto conductive glass slides and a layer of MALDI matrix is applied, typically by aerosol. Defined areas of measurement are established by the user to using a digital image of the sample. The size of the virtual grid establishes the image resolution and is tailored for the experimental question to be answered.

Ultraflextreme MALDI

Agilent 6550 LC-Qq-ToF MS

The 6550 LC-Qq-ToF MS provides high accurate mass analysis of complex samples. Agilent’s proprietary iFunnel technology combines the high-efficiency ESI ion generation and focusing of Agilent Jet Stream sample introduction with unique hexabore sampling capillary and dual stage ion funnel assemblies. Agilent iFunnel technology provides a level of robustness unmatched in the industry by combining true orthogonal electrospray orientation with a heated, off-axis funnel geometry to prevent transmission of uncharged species. The approach offers qualitative and quantitative characterization of samples, including metabolites, lipids, and peptides. The system is supported by Agilent’s MassHunter software to allow streamlined metabolite and lipid identification. The system can be configured for low (nano) or high (analytical) flow rates to deal with various sample types and amounts.

Agilent QTOF 6545.png

Thermo Orbitrap Exploris 480 MS

The Orbitrap Exploris 480 mass spectrometer joins the family of next-generation Thermo Scientific mass spectrometers built on a common architecture with the guiding principle of ultimate performance and ease of use. Combined with the Thermo UltiMate RSLC nanoLC and ProtemeDiscoverer software, the platform is the latest generation of accurate mass, high resolution technology for proteomics analysis. The system is configured for label-free nLC-MSMS of complex protein samples and can be used for high-throughput characterization of serum, plasma, and other complex sample types. The system is equipped with the Thermo Scientific™ FAIMS Pro™ interface, which minimizes co-isolation of isobaric peptides, increases the number of unique peptides identified, and improves signal-to-noise to sample low-abundance peptides.

Thermo Orbitrap

Bruker timsTOF fleX MS

The timsTOF fleX MS system is a super-fast and highly sensitive ESI instrument for all of your Omics analyses with an integrated MALDI source for rapid MALDI Imaging. Built on the standard for shotgun proteomics, the timsTOF fleX combines the best in 4D-Omics using robust ESI measurements, along with Bruker’s cutting edge MALDI Imaging technology to spatially resolve a wide range of molecules directly from tissue, all in one single platform. Changing from ESI to MALDI occurs within a matter of seconds. No complicated changeover means making zero compromises in productivity and the ability to move effortlessly from world class Omics identification and quantification workflows to creating high-definition molecular maps of tissue sections to visualise your analyte of target.

Bruker timeTOF flex machine

Pricing and Quotation

The instrument access fee schedule below is subject to change. Pricing is reviewed on an annual basis. Although every effort is made to update the information below, please contact MSP directly for a formal quote.

Formal quotes are available upon request via the MSP Quotation Request Form. Please email the filled out form along with any extra details to msp.bioplatforms@unisa.edu.au 

 

Please download our MSP Facility Information Handbook for more information.

NOTE: For all South Australian Universities and Institutes, the internal rates apply.

Main Services

Effective 01 January 2020

MSP Pricing Structure Main Services

Quantitative Proteomics

MSP Pricing Structure Quantitative Proteomics

* All prices above are excluding GST. Prices include UniSA Levies where applicable. Formal quotes are available upon request via email

Some instruments have a 1 hour minimum booking time.

Technician time may be charged to external and industry users in addition to access costs. Typical Technical charges:

  • Training: $170 / hour
  • Technical services: $170 / hour

Repairs due to instrument damage resulting from operation without due care and attention will be charged to the user's organisation.

Contact details

UniSA HB Building

Location Level 8, HB Building Cnr North Terrace and Morphett Street, Adelaide 5000

The University of South Australia, the State and Federal Governments and Bioplatforms Australia enabled the Mass Spectrometry and Proteomics facility at the University of South Australia

UniSA logo

Government of SA logo

 

Bioplatforms Australia logo