Experimental Design and Analysis
Over the course of a few years the Biometrics Group of the PBRC have developed a number of R-based codes related to the design of high throughput plant experiments. These are available through CRAN (note that they are not standalone pieces of software) via the following links.
RootAnalyzer, developed at the PBRC, is a fully automated tool for efficiently extracting and analyzing anatomical traits from root cross section images. Using a range of image processing techniques such as local thresholding and nearest neighbor identification, RootAnalyzer segments the plant root from the image's background, classifies and characterizes the cortex, stele, endodermis and metaxylem, and subsequently produces statistics about the morphological properties of the root cells and tissues.
RootAnalyzer is available to download at http://sourceforge.net/projects/rootanalyzer/
RootGraph: The software tool represents a numerical scheme for accurate, detailed and high-throughput analysis of scanned images of plant roots. The tool is a fully automated and robust approach for the detailed characterization of root traits, based on a graph optimization process. The scheme, firstly, distinguishes primary roots from lateral roots and, secondly, quantifies a broad spectrum of root traits for each identified primary and lateral root. The program associates lateral roots and their properties with the specific primary root from which the laterals emerge. The program’s performance has compared favourably against results based on manual measurements.
RootGraph is available to download at http://www.plant-image-analysis.org/software/rootgraph
Cell Mobility is a program to simulate the dynamics of charged particles moving under an oscillating electric field. It calculates the
- dynamic mobility
- electrical conductivity, and
of concentrated charged colloidal systems.
For more information and to purchase this software please contact Professor Stan Miklavcic
Sequencing error correction without a reference genome
This code provides a method for sequencing error correction for Illumina next generation sequencing data sets, when a reference genome is not available. It is available for download here.
This work is described in the paper:
Julie A Sleep, Andreas W Schreiber and Ute Baumann, Sequencing error correction without a reference genome, BMC Bioinformatics, 14:367, 2013.
Please contact Ms Julie Sleep for more information.