Sequencing of crop genomes provides deep insights into plant evolution and new tools for crop improvement. The sequencing of giant and repetitive genomes such as wheat and barley can be a challenge which requires worldwide collaborative efforts.
A preliminary step to genome sequencing is the physical mapping, the construction of a scaffold of DNA segments where genes are positioned. Our project is working towards development of a physical map and ultimately a full genome sequence for barley chromosome 7H and wheat chromosomes 7A, 7B and 7D. These chromosomes carry loci controlling important traits including yield, quality, disease resistance and abiotic-stress tolerance. We evaluate the physical distance between markers along these chromosomes, assess their gene content and analyse how the genes recombine between parental lines for breeding of new varieties. Ultimately new genes will be discovered in wheat by shotgun sequencing of isolated chromosome arms using next-generation sequencing technologies.
We also aim to develop computational models describing relationships between genomes of wheat, barley and other cereals. This would enable the use of simpler genomes such as barley to accelerate assembly of the maps in corresponding regions of the more complex wheat genome.
- Ute Baumann
- Delphine Fleury
- Peter Langridge
- Andreas Schreiber
- Jaroslav Dolezel, Institute of Experimental Botany
- David Edwards, Australian Centre for Plant Functional Genomics
- Matthew Hayden, DPI Victorian AgriBiosciences Center
- Nils Stein, Leibniz Institute of Plant Genetics and Crop Plant Research